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KMID : 1134820230520020203
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Journal of the Korean Society of Food Science and Nutrition
2023 Volume.52 No. 2 p.203 ~ p.209
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Enhancement of Collagen Synthesis through the Regulations of MMP-1, COL1A1, and SMAD-3 by Extract of the Freshwater Microalgae Mychonastes sp. 249
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Hong Ji-Woo
Yeom Suh-Hee
Oh Ye-Sol
Park Jae-Hoon
Kang Min-Ho
Kim Yeong-Hyeon
Kim Z-Hun
Yim Kyung-June
Kim Jin-Woo
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Abstract
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In this study, we investigated the effect of enhanced collagen synthesis by Mychonastes sp. 249 (a type of freshwater microalgae) extract (MSE) on skin regeneration. The effect of MSE on human dermal fibroblast (HDF) migration was studied to confirm its skin regenerative effect, and at 1.0 mg/mL MSE increased HDF cell migration by 21.2%. To study the mechanism responsible, the mRNA levels of MMP-1 (matrix metalloproteinase-1), COL1A1 (collagen type I alpha 1), and SMAD-3 (suppressor of mothers against decapentaplegic-3) were evaluated by RT-PCR. The results showed that in the concentration range 0.0 to 1.0 mg/mL, MSE dose-dependently affected mRNA expressions in HDF cells (P<0.05). LC-MS/MS analysis identified eriodictyol (a flavonoid) as the main component of MSE. These results suggest that MSE could be used as a skin regeneration agent that inhibits collagen decomposition in HDF cells.
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KEYWORD
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collagen synthesis, flavonoid, freshwater microalgae, Mychonastes sp. 249, skin regeneration
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